Ulyanovsk Medico-biological Journal

BIOLOGICAL SCIENCES

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DOI 10.34014/2227-1848-2025-2-133-140

T-LYMPHOCYTES IN THE GREATER OMENTUM IN SEROUS BORDERLINE OVARIAN TUMORS

E.S. Gozman

Kirov State Medical University, Ministry of Health of the Russian Federation, Kirov, Russia

 

The aim of the study is to describe the subpopulations of CD4+, CD8+ T-lymphocytes of the greater omentum in serous borderline ovarian tumor.

Materials and Methods. The immunohistochemical method was used to analyze the markers of CD4, CD8 T-lymphocytes in the greater omentum in 30 patients. In a research study, inclusion criteria were: the serous borderline ovarian tumor (with and without implants), surgeries for borderline ovarian tumor with omentectomy, age 20-45 years old. At the same time, severe concomitant diseases served as an exclusion criterion. The authors conducted morphometric assessment and analyzed images. STATISTICA 10 (StatSoft) program was used for statistical processing of the results.

Results: Evaluation of CD4 and CD8 expression levels in the greater omentum demonstrated that it was higher in serous borderline tumor without implants compared to tumor with implants.

Conclusion. The greater omentum is involved in immune reactions due to the T-lymphocyte population.

Key words: ovarian tumor, borderline ovarian tumor, greater omentum, T-lymphocytes.

 

Conflict of interest. The author declares no conflict of interest.

 

References

  1. Perween R. Borderline Ovarian Tumor – An Overview and Evidence Based Management. Pan Asian Journal Obs Gyn. 2019; 2 (1): 31–35.

  2. Havrlentova L., Faistova H., Mazur M., Ziak D., Polak S. Comparative analysis of human omental milky spots between the patients with colon cancer and the control group. Bratisl. Lek. Listy. 2017; 118 (10): 580–584.

  3. Liu J., Li Y., Geng X. Milkyspots: omental functional units and hotbeds for peritoneal cancer metastasis. Tumor Biol. 2016; 37: 5716–5725.

  4. Conroy M.J., Melo A.M., Maher S.G., Doyle S.L., Foley E. Identifying a novel role for Fractalkine (CX3CL1) in memory CD8+ T cell accumulation in the omentum of obesity-associated cancer patients. Front. Immunol. 2018; 9: 1867–1868. DOI: 10.3389/fimmu.2018.01867.

  5. Cipolletta D., Cohen P., Spiegelman B.M., Benoist C., Mathis D. Appearance and disappearance of the mRNA signature characteristic of Treg cells in visceral adipose tissue: age, diet, and PPARγ effects. Proc. Natl. Acad. 2015; 112 (2): 482–487.

  6. Ke X. Targeting cytokines secreted by CD4+ CD25(high) CD127(low) regulatory T cells inhibits ovarian cancer progression. Scandinavian Journal of Immunol. 2019; 89 (2): 32–35.

  7. Boucher P., Gotthardt M., Li W.P., Anderson R.G., Herz J. Science. LRP: role in vascular wall integrity and protection from atherosclerosis. Science. 2003; 300 (5617): 329–332.

  8. Cleypool C., Bleys R., Schurink B. Morphological hallmarks facilitating distinction of omental milky spots and lymph nodes: an exploratory study on their discriminative capacity. Histopathology. 2020; 4: 18–25.

  9. Brifault C., Gilder A.S., Laudati E., Banki M., Gonias S.L. Shedding of membrane-associated LDL receptorrelated protein-1 from microglia amplifies and sustains neuroinflammation. J. Biol. Chem. 2017; 292 (45): 18699–18712.

  10. Liu M., Meza-Perez S., Randall T. Specialized immune responses in the peritoneal cavity and omentum. Jourmal of Leukocyte Biology. 2021; 109 ( 4): 718–728. DOI: 10.1002/JLB.5MIR0720-271RR.

  11. Wouters M., Workel H., Komdeur F. Treatment regimen, surgical outcome, and T-cell differentiation influence prognostic benefit of tumor-infiltrating lymphocytes in high-grade serous ovarian cancer. Clin. Cancer Research. 2016; 22 (3): 715–723. DOI: 10.1158/1078-0432.CCR-15.

  12. Cai L., Wang Z., Ji A., Meyer J.M., van der Westhuyzen D.R. Scavenger receptor CD36 expression contributes to adipose tissue inflammation and cell death in diet-induced obesity. PLoS ONE. 2012; 7 (5): e36785. DOI: 10.1371/journal.pone.0036785.

  13. Ren K., Xie X., Min T., Sun T., Wang H., Zhang Y., Zhang H. Development of the peritoneal metastasis: a review of back-grounds, mechanisms, treatments and prospects. Jourmal Clin Med. 2022; 12 (1): 103–105. DOI: 10.3390/jcm12010103.

  14. Received April 15, 2025; accepted May 12, 2025.

  15. Information about the authors

  16. Gozman Elena Sergeevna, Postgraduate Student, Chair of Histology, Embryology and Cytology, Kirov State Medical University, Ministry of Health of the Russian Federation. 610998, Russia, Kirov, K. Marx St., 112; e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., ORCID ID: http://orcid.org/0009-0006-6516-0322.

  17. For citation

  18. Gozman E.S. T-limfotsity bol'shogo sal'nika pri seroznykh pogranichnykh opukholyakh yaichnikov [T-lymphocytes of the greater omentum in serous borderline ovarian tumors]. Ul'yanovskiy mediko-biologicheskiy zhurnal. 2025; 2: 133–140. DOI: 10.34014/2227-1848-2025-2-133-140 (in Russian). 

Received April 15, 2025; accepted May 12, 2025.

Information about the authors

Gozman Elena Sergeevna, Postgraduate Student, Chair of Histology, Embryology and Cytology, Kirov State Medical University, Ministry of Health of the Russian Federation. 610998, Russia, Kirov, K. Marx St., 112; e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., ORCID ID: http://orcid.org/0009-0006-6516-0322

 

For citation

Gozman E.S. T-limfotsity bol'shogo sal'nika pri seroznykh pogranichnykh opukholyakh yaichnikov [T-lymphocytes of the greater omentum in serous borderline ovarian tumors]. Ul'yanovskiy mediko-biologicheskiy zhurnal. 2025; 2: 133–140. DOI: 10.34014/2227-1848-2025-2-133-140 (in Russian).

 

 

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УДК 618.11-006:616.382.1:612.017.1

DOI 10.34014/2227-1848-2025-2-133-140

Т-ЛИМФОЦИТЫ БОЛЬШОГО САЛЬНИКА ПРИ СЕРОЗНЫХ ПОГРАНИЧНЫХ ОПУХОЛЯХ ЯИЧНИКОВ

Е.С. Гозман

ФГБОУ ВО «Кировский государственный медицинский университет» Министерства здравоохранения Российской Федерации, г. Киров, Россия

 

Цель. Описать субпопуляции Т-лимфоцитов CD4+, CD8+ большого сальника при серозной пограничной опухоли яичников.

Материалы и методы. Иммуногистохимическим методом изучались маркеры Т-лимфоцитов CD4, CD8 большого сальника 30 пациенток. Критерии включения в исследование: наличие серозной пограничной опухоли яичников (с имплантами и без имплантов), хирургическое лечение пограничной опухоли яичников с оментэктомией, возраст от 20 до 45 лет. Критерии исключения: тяжелые сопутствующие заболевания. Проводились морфометрическая оценка, анализ фотоснимков, статистическая обработка полученных результатов при помощи программы STATISTICA 10 (StatSoft).

Результаты. Оценка уровней экспрессии CD4 и CD8 в больших сальниках продемонстрировала, что при серозной пограничной опухоли без имплантов она была выше по сравнению с опухолью с имплантами.

Выводы. Большой сальник принимает участие в осуществлении иммунных реакций за счет популяции Т-лимфоцитов.

Ключевые слова: опухоль яичника, пограничная опухоль яичника, большой сальник, Т-лимфоциты.

 

Конфликт интересов. Автор заявляет об отсутствии конфликта интересов.

 

Литература

  1. Perween R. Borderline Ovarian Tumor – An Overview and Evidence Based Management. Pan Asian Journal Obs Gyn. 2019; 2 (1): 31–35.

  2. Havrlentova L., Faistova H., Mazur M., Ziak D., Polak S. Comparative analysis of human omental milky spots between the patients with colon cancer and the control group. Bratisl. Lek. Listy. 2017; 118 (10): 580–584.

  3. Liu J., Li Y., Geng X. Milkyspots: omental functional units and hotbeds for peritoneal cancer metastasis. Tumor Biol. 2016; 37: 5716–5725.

  4. Conroy M.J., Melo A.M., Maher S.G., Doyle S.L., Foley E. Identifying a novel role for Fractalkine (CX3CL1) in memory CD8+ T cell accumulation in the omentum of obesity-associated cancer patients. Front. Immunol. 2018; 9: 1867–1868. DOI: 10.3389/fimmu.2018.01867.

  5. Cipolletta D., Cohen P., Spiegelman B.M., Benoist C., Mathis D. Appearance and disappearance of the mRNA signature characteristic of Treg cells in visceral adipose tissue: age, diet, and PPARγ effects. Proc. Natl. Acad. 2015; 112 (2): 482–487.

  6. Ke X. Targeting cytokines secreted by CD4+ CD25(high) CD127(low) regulatory T cells inhibits ovarian cancer progression. Scandinavian Journal of Immunol. 2019; 89 (2): 32–35.

  7. Boucher P., Gotthardt M., Li W.P., Anderson R.G., Herz J. Science. LRP: role in vascular wall integrity and protection from atherosclerosis. Science. 2003; 300 (5617): 329–332.

  8. Cleypool C., Bleys R., Schurink B. Morphological hallmarks facilitating distinction of omental milky spots and lymph nodes: an exploratory study on their discriminative capacity. Histopathology. 2020; 4: 18–25.

  9. Brifault C., Gilder A.S., Laudati E., Banki M., Gonias S.L. Shedding of membrane-associated LDL receptorrelated protein-1 from microglia amplifies and sustains neuroinflammation. J. Biol. Chem. 2017; 292 (45): 18699–18712.

  10. Liu M., Meza-Perez S., Randall T. Specialized immune responses in the peritoneal cavity and omentum. Jourmal of Leukocyte Biology. 2021; 109 ( 4): 718–728. DOI: 10.1002/JLB.5MIR0720-271RR.

  11. Wouters M., Workel H., Komdeur F. Treatment regimen, surgical outcome, and T-cell differentiation influence prognostic benefit of tumor-infiltrating lymphocytes in high-grade serous ovarian cancer. Clin. Cancer Research. 2016; 22 (3): 715–723. DOI: 10.1158/1078-0432.CCR-15.

  12. Cai L., Wang Z., Ji A., Meyer J.M., van der Westhuyzen D.R. Scavenger receptor CD36 expression contributes to adipose tissue inflammation and cell death in diet-induced obesity. PLoS ONE. 2012; 7 (5): e36785. DOI: 10.1371/journal.pone.0036785.

  13. Ren K., Xie X., Min T., Sun T., Wang H., Zhang Y., Zhang H. Development of the peritoneal metastasis: a review of back-grounds, mechanisms, treatments and prospects. Jourmal Clin Med. 2022; 12 (1): 103–105. DOI: 10.3390/jcm12010103.

Поступила в редакцию 15.04.2025; принята 12.05.2025.

 

Автор

Гозман Елена Сергеевна – аспирант кафедры гистологии, эмбриологии и цитологии, ФГБОУ ВО «Кировский государственный медицинский университет» Министерства здравоохранения Российской Федерации. 610998, Россия, г. Киров, ул. К. Маркса, 112; e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., ORCID ID: http://orcid.org/0009-0006-6516-0322

 

Образец цитирования

Гозман Е.С. Т-лимфоциты большого сальника при серозных пограничных опухолях яичников. Ульяновский медико-биологический журнал. 2025; 2: 133–140. DOI: 10.34014/2227-1848-2025-2-133-140.